抄録
The liver regeneration after injury is is regulated by various growth factors and cytokines. Release of these growth factors is deeply related to degradation of extracellular matrix (ECM). The ECM degradation is regulated by the activation of plasminogen (Plg) and matrix metalloproteinase (MMP) systems. The liver regenerations on knockout mice (KO) for fibrinolytic factors were examined by using CCl4 injection model. Then, the relationship between the liver regeneration and plasmin/α2-AP system was analyzed by using the hepatocytes isolated from mouse liver. The proliferation ability of the hepatocytes isolated from mice without CCl4 injection showed similar in all genotype mice. On the other hand, the proliferation ability of the hepatocytes from mice of 5 days after CCl4 injection was significantly increased in the α2-APKO as compared to the WT, but decreased in the PlgKO, and Plg-α2-APKO. The hepatocytes isolated from all genotype mice similarly bound to the immobilized Plg. The binding ability of hepatocytes, which were isolated from mouse after CCl4 injection, to Plg was significantly decreased in the PlgKO, and Plg-α2-APKO as compared to the WT, or α2-APKO. The activation of Plg was significantly increased in the presence of mouse hepatocytes. The plasmin inhibition by α2-AP in the presence of mouse hepatocytes was weaker than that in the absence of mouse hepatocytes. These results indicate that the plasmin/α2-AP system on the surfaces of mouse hepatocytes plays important roles in liver regeneration. [Jpn J Physiol 54 Suppl:S114 (2004)]
