抄録
Effects of a time-varying magnetic field on signal transduction of cultured bovine adrenal chromaffin cells. Ikehara, T.1, Teramoto, T.2, Houchi, H.2, Yamaguchi, H.4, Hosokawa, K.1, Kinouchi, Y.3, Yoshizaki, K.1 and Miyamoto, H.11Dept. Physiol., 2Dept. Pharmacy, Sch. of Med., 3Dept. Electric. & Electronic Engr., Fac. Engr., Univ. Tokushima, Tokushima 770-8503, 4Dept of Environ. Physiol., Fac. Human Life Sci., Tokushima Bunri Univ., Tokushima 770-8513, Japan. Effects of a time-varying magnetic field on chromaffin cells were examined. The magnetic flux density was varied intermittently from 0.07 -1.5 T at an interval of 3 sec. An increase in intracellular Ca2+ concentration ([Ca2+]i) was stimulated by addition of bradykinin or acetylcholine (ACh) to a medium. The stimulator-induced increase in [Ca2+]i in Ca2+-free medium was inhibited by 2 hr exposure to the magnetic field. In normal medium, the exposure did not affect the ACh-induced increase, but slowed the decay phase of [Ca2+]i after the peak. Also, the exposure affected the cellular ATP content and oxygen uptake. These results suggest that exposure to the magnetic field affect not only ER but also other organells, and the influences by the exposure on these organells might suppress the increase in [Ca2+]i. [Jpn J Physiol 54 Suppl:S65 (2004)]
