日本生理学会大会発表要旨集
日本生理学会大会発表要旨集
セッションID: 1S07H4
会議情報
The state of the art in smooth muscle research in the post-genomic era
機能的プロテオミクスとRNA干渉を用いた血管平滑筋のシグナル伝達研究
小林 誠岸 博子川道 穂津美苗 俊英加治屋 勝子
著者情報
会議録・要旨集 フリー

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抄録
While Ca2+-dependent contraction of vascular smooth muscle (VSM) regulates physiological vascular tone, the Rho-kinase (ROK)-mediated Ca2+-sensitization of VSM contraction plays a pivotal role in abnormal VSM contraction such as vasospasm. As an upstream mediator for such abnormal Ca2+-sensitizing pathway, we previously identified sphingosylphosphorylcholine (SPC), which induces the Ca2+-sensitization through the activation of ROK, which was blocked by dominant negative ROK. Inhibitors of Src family tyrosine kinases (Src-TKs) blocked the SPC-induced contraction and activation of ROK and tyrosine phosphorylation of several proteins, suggesting the involvement of a Src-TK-mediated tyrosine phosphorylation. Furthermore, using the selective knockdown of the target molecule by siRNA, we determined that Fyn, a member of Src-TK, mediates the SPC-induced ROK-mediated Ca2+-sensitization of VSM contraction. Subsequently, in order to screen for the molecular target(s) downstream of Fyn, we performed mass spectrometry of possible signal molecules whose tyrosine was phosphorylated by a SPC/Fyn pathway, and identified p160 and p40. Further characterization and roles of these molecules are currently performed and some of the results will be presented. [Jpn J Physiol 55 Suppl:S16 (2005)]
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© 2005 日本生理学会
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