抄録
[Purpose] The stereoisomers of D-glucoses are expected to have many physiological activities because of the similarities of the structures, though most of these are rarely found in the nature. Angiogenesis plays an important role in tumor growth and metastasis. To estimate the applicability of monosaccharides to anti-cancer drugs, we examined the effects of monosaccharides on the proliferation, the tube formation and the glucose uptake of human umbilical vein endothelial cells (HUVEC). Thirty six kinds of monosaccharides were used and the data were correlated with their structures. [Methods and Results] The proliferation assay was performed 48h after the addition of sugars (0–20 mM) to the culture medium. Seven sugars were found to inhibit the proliferation in a dose dependent manner. The tube formation assay was performed by CD31 staining of endothelial cells 10 days after the addition of sugars (0–20 mM) to the co-culture system of HUVEC and human fibroblast. Fourteen sugars inhibited the tube formation in a dose dependent manner. To estimate the glucose uptake, 14C labeled D-glucose was added to the culture medium with and without other sugars, and the uptake was calculated at 48 h. Three sugars inhibited the glucose uptake in HUVEC. [Conclusion] Comparing the stable structure of the sugars, following common structures seem to be required to inhibit the proliferation or tube formation of HUVEC: (ii) Less 1,3-diaxial interaction in the pyranose form: (ii) Equatorial substituent at C2 or C3 is H (hydrogen). [Jpn J Physiol 55 Suppl:S74 (2005)]
