抄録
Human umbilical endothelial cells (HUVECs) show various responses including morphological changes and protein expressions in response to mechanical stretch. Our previous studies revealed that intracellular Ca increase in response to mechanical stretch via Ca permeable stretch-activated (SA) channel activation is critical in HUVECs cultured on an elastic PDMS (polydimethylsiloxane) membrane. Since recent reports suggest that the transient receptor potential (TRP) channels may form the SA channel, we investigated the involvement of TRPV2 in the stretch-induced Ca increase in HUVECs. Human TRPV2 was isolate from a HUVEC cDNA library. Heterologous expression of the human TRPV2 in COS7 cells resulted in the stretch-induced Ca increase and injection of a TRPV2-specific siRNA in HUVECs abolished the stretch-induced Ca increase.These observations indicate that TRPV2 plays a critical role in the stretch-induced Ca increase in HUVECs. [J Physiol Sci. 2006;56 Suppl:S48]
