Mammalian inferior colliculus(IC) is an integrative auditory processing center of the midbrain. IC contains a large amount of GABAergic neurons and forms complex neural circuits. We investigated the response properties of IC neurons to sounds and their morphological characteristics by juxtacelluler staining method. Further, by GAD-67 immunohistological stainig, we classified the recorded neurons into two groups, GABAergic and non-GABAergic neurons, and compared the response properties to sound stimuli. [J Physiol Sci. 2007;57 Suppl:S101]