抄録
We developed a simple learning and memory test on the basis of repetitive counting locomotion activity and rearing for one hour in an open field. Here we tested whether the causal relationship between brain chemical elements and administrated Pb-induced behavioral changes can be detected. Correlation analysis was applied for 4320 pairs; 6 brain areas that are reportedly involved in learning, memory, and emotion (prefrontal cortex, nucleus accumbens, amygdala, dorsal hippocampus, ventral hippocampus, and cerebellum punched or cut out from brain slices) x (15 chemical elements [Mg, Al, P, Ca, Mn, Fe, Co, Ni, Cu, Zn, Rb, Sr, Ag, Ba, and Pb] measured by Inductively Coupled Plasma Mass Spectroscopy) x behavioral data (locomotor activity and rearing). We detected the significance of brain area-specific positive and negative correlations between chemical elements themselves then between chemical elements and the behavioral indexes of our developed method, which seems to be practical and useful for the initial behavioral test of learning and memory regarding developmental neurotoxicology. [J Physiol Sci. 2007;57 Suppl:S166]
