抄録
There is much evidence that the reactive oxygen species(ROS) affect several types of diseases. Although bisphosphonates, such as etidronate and alendronate(AR),are frequently used for the treatment of osteoporosis, the influence of bisphosphonate on the production of ROS is not fully understood. The present study, therefore, was designed to examine the influence of bisphosphonate, especially AR, on the production of ROS in vitro and in vivo. The first set of experiments was carried out to examine the in vitro effect of AR on the production of ROS by examining the ability of fibroblasts from rats with adjuvant arthritis to produce nitric oxide in response to lipopolysaccharide(LPS)stimulation.It caused suppression of NO production from fibroblasts and the minimum dose of AR was 10.0ng/ml.The second part of experiments were designed to examine whether the suppressive activity of AR on ROS production in vivo was observed in vitro by measuring hydroperoxide contents in plasma. Thus, adjuvant arthritis in Lewis rats were injected intraperitoneally with various doses of AR once a day for 3 weeks.Daily intraperitoneally with various doses of AR once a day for 3 weeks,starting one week after adjuvant injection. Daily intraperitoneal administration of AR at more than 0.5ng/kg for 3weeks could cause the significant suppression of hydroperoxide formation in plasma. These results may suggest that AR could suppress the production of reactive oxygen species and results in attenuation of the clinical conditions of disease with bone destruction. [J Physiol Sci. 2007;57 Suppl:S244]
