抄録
Most GABAergic interneurons of the rodent cortex originate from the ganglionic eminences (GEs). During perinatal days, they migrate tangentially through the marginal zone (MZ) and intermediate zone/subventricular zone (IZ/SVZ) towards the cortex. After reaching the cortex, they migrate into the cortical plate and incorporate into their appropriate laminar position. To investigate the dynamics of the migrating interneurons in living immature animal, we have developed in vivo time-lapse imaging with two-photon microscopy employed on immature transgenic mice expressing Venus in interneurons. At first, to establish the time-lapse imaging of immature mouse neocortex neurons, we designed for the specialized experimental preparations. For example, we developed four-direction restraint bars (front, back, left, and right side of the head) to alleviate heartbeat and breathing which are suitable for immature mouse. Before time-lapse imaging, to identify Venus labeling neurons, we tried double-labeling immunofluorescent staining against Venus fluorescence and GABA at P60 mouse. In result, GABA staining was mostly observed in Venus positive cells (more than 90%) in neocortex. In combination with these new methods with two-photon microscopy, we could observe the multidirectional tangential migration of GABAergic interneurons in the MZ in vivo. This approach will be a tool for understand neural activity in developing intact brain. [J Physiol Sci. 2008;58 Suppl:S125]
