抄録
AAV vectors have gained a preeminent position in the field of gene delivery to the brain through their ability to achieve extensive and long-term transduction of neurons with no apparent toxicity. Although various AAV serotypes have recently become available, the transduction patterns of AAV serotypes within the hippocampal neurons have not yet been clarified. In this study, we set out to compare the expression patterns of enhanced green fluorescent protein (EGFP) in the adult mouse hippocampus by using titer-matched AAV vectors. Recombinant AAV serotypes 2, 8, and 9 incorporating chicken beta-actin (CAG) promoter-driven EGFP expression cassette were purified by an ion-exchange chromatography. By biophotonic imaging, the neuronal type affected viral tropism and transgene distribution. The EGFP expression levels with AAV8 were greater than that with the recombinant AAV2 or AAV9 in pyramidal neurons and granule cells, whereas interneurons were inefficiently transduced with the AAV8 vector. These results suggest that vectors based on distinct AAV serotypes can be adequately chosen for specific applications in the hippocampal neurons. [J Physiol Sci. 2008;58 Suppl:S129]
