抄録
Growth of prostatic epithelial cells is androgen-dependent, however the mechanism of androgen action on the progression of cell cycle is not fully defined. We investigated whether the androgen-dependent prostatic epithelial cell growth is mediated by androgen regulation of expression and activation of cell cycle associated proteins. For this purpose, we used androgen-dependent prostatic cancer cell line, LNCaP-FGC. We found that the proliferation of LNCaP cell was significantly arrested in G1/S and G2/M phases after treatment with the charcoal-dextran stripped serum-containing medium. Dehydrotestosterone (DHT) at low concentrations (0.001 to 0.1 nM) stimulates cell growth dose-dependently. However, DHT at high concentrations (1.0 to 100 nM) inhibits cell proliferation by arresting the cell cycle only in the G1 phase. To clarify the molecular mechanisms causing these differences, we studied the influence of DHT on several cell cycle signaling molecules. The effects of DHT on major cell-cycle regulators such as cyclin-dependent kinase inhibitors (CDIs), cyclin-dependent kinases (CDKs) and their downstream signaling molecules will be discussed. [J Physiol Sci. 2008;58 Suppl:S207]
