Abstract
59Fe-Resin strip adsorption technique for the measurement of unsaturated iron binding capacity (UIBC) in serum was compared with the chemical colorimetric method. The effect of some anticoagulants against UIBC value were also studied. The conclusions were as follows:
1) 59Fe-Resin strip adsorption method was easy to perform, and it required less amount of sample serum, than in other method.
2) When incubation temperature was raised to 37°C, the iron adsorption rate in the resin strip was increased. This made the whole procedure in shorter period of time.
3) Either resin activation by HCl or by HCl and NaOH, gave little on the iron adsorption.
4) Once combined with EDTA salt, the iron was not adsorbed by the resin strip. UIBC values were influenced by the use of other anticoagulants.
