Abstract
A comparative study of whole-body autoradio grams was carried out to estimate the effect of difference in the labelling radionuclides on the evaluation of autoradio grams. Two major factors involved are the release of labelling nuclide in the body and the energy intensity of β-rays which influence the resolution in autoradoigram.
Three test substances, i.e., 131I-5-chloro-7-iodo-8-hydroxy-quinoline (Quinoform), Quinoform-2, 3, 4-14C and Na131I, were injected to mice, and the whole-body autoradio-grams were compared in detail with each other.
The organs to be checked as for the release of 131I were found to be the thyroid gland, salivary gland, seminal vesicle, content of the stomach, and skin. Based on these results a discussion was made of the possibility that the release of 131I from a compound can be checked in general by a comparative examination of the whole-body autoradiograms with different radionuclides.
Additional studies showed that the removal of an emulsion layer from one side of X-ray film is an effective and practical way to improve the resolution in autoradiogram.
