A Study on the Insulin Radioimmunoassay with Sephadex-bound Antibody

Abstract

A method of insulin radioimmunoassay with Sephadex-bound antibody (Phadebas^® Insulin Test) was tested for the determination of serum insulin.
The time to reach the maximal binding rate of insulin to the solid phase antibody was not so different to that of two antibody method. But enough accuracy for the practical purposes was obtained in overnight incubation.
The insulin values obtained with this method was well in accord with that with the two antibody method in good conditions, and was not interfered by the presence of serum proteins, judged from the results of recovery test.
Though this method was a little less sensitive and accurate as compared to the two antibody method, this method was simple and convenient and has enough sensitivity and reliability for the usual routine serum insulin assay.