Comparison of Phadebas Insulin Test and the Ethanol Precipitation

Method in Radioimmunoassay of Plasma Insulin

Abstract

Ninety-five plasma samples were assayed for insulin using Phadebas Insulin Test (an insulin radioimmunoassay kit: manufactured by Pharmacia Co., Ltd.) in comparison with the results obtained by the ethanol precipitation method.
The correlation between two methods was high (r=0.911, p<0.001), and the regression coefficient was Y6.848+0.821X.
From the results obtained, the Phadebas Insulin Test can be used as a practically useful method for radioimmunoassay of plasma insulin.