Abstract
Usefulness of125I column triiodothyronine test, “Trilute”, measuring thyroid function indirectly by indicating the available thyroxine binding globulin sites was evaluated. Fundamental studies—
Incubation time: This kit was considered to be convenient to determine many specimens at a time as there was no effect of incubation time on the results.
Incubation temperature: Determination should be carried on at the room temperature of 25°C or higher, as the deviation by temperature correction is small at this temperature. A satisfactory reproducibiliy of the kit was obtained in measuring the sera from the subjects with various thyroid functions and from the pregnant women.
Clinical studies—
In normal subjects, the Trilute value ranged 38.14 to 65.70, without overlap with those of hyperthyroid and pregnant, but with considerable overlap with that of hypothyroid. A good correlation was observed between the values of Triosorb resin sponge uptake and that of Trilute. The values of Tetrasorb and Trilute were significantly correlated, with a exception of pregnant women, who showed higher value of Tetrasorb and lower value of Trilute.
The Trilute test could be carried on simply and easily, with careful pipetting 0.05ml of serum specimen and with controlling the incubation temperature around 25°C, and was found to be useful routine examination of thyroid function.
