RADIOISOTOPES

Fundamental and Clinical Studies of “Trilute” for Thyroid Function

Tadako KOUSAKA, Toru MORI, Ken HAMAMOTO, Yosuke TAKEDA, Katsuji IKEKUB ...

1974Volume 23Issue 6 Pages 329-334
Published: June 15, 1974
Released on J-STAGE: September 07, 2010

DOIhttps://doi.org/10.3769/radioisotopes.23.6_329

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Usefulness of¹²⁵I column triiodothyronine test, “Trilute”, measuring thyroid function indirectly by indicating the available thyroxine binding globulin sites was evaluated. Fundamental studies—
Incubation time: This kit was considered to be convenient to determine many specimens at a time as there was no effect of incubation time on the results.
Incubation temperature: Determination should be carried on at the room temperature of 25°C or higher, as the deviation by temperature correction is small at this temperature. A satisfactory reproducibiliy of the kit was obtained in measuring the sera from the subjects with various thyroid functions and from the pregnant women.
Clinical studies—
In normal subjects, the Trilute value ranged 38.14 to 65.70, without overlap with those of hyperthyroid and pregnant, but with considerable overlap with that of hypothyroid. A good correlation was observed between the values of Triosorb resin sponge uptake and that of Trilute. The values of Tetrasorb and Trilute were significantly correlated, with a exception of pregnant women, who showed higher value of Tetrasorb and lower value of Trilute.
The Trilute test could be carried on simply and easily, with careful pipetting 0.05ml of serum specimen and with controlling the incubation temperature around 25°C, and was found to be useful routine examination of thyroid function.

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Fundamental and Clinical Studies of “Tetralute” for Thyroid Function

Tadako KOUSAKA, Toru MORI, Ken HAMAMOTO, Yosuke TAKEDA, Katsuji IKEKUB ...

1974Volume 23Issue 6 Pages 335-341
Published: June 15, 1974
Released on J-STAGE: September 07, 2010

DOIhttps://doi.org/10.3769/radioisotopes.23.6_335

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Usefulness of Tetralute kit for measuring total serum thyroxine was evaluated.
The characteristics and advantages of this method, compared with other in vitro thyroxine measurements were as follows.
1) Virtually all thyroxine is extracted from the serum by placing it in a strong alkaline Sephadex G-25 column. This procedure can be carried on easily in a short time.
2) Only 0.1 ml of serum specimen is used in this measurement.
3) Such an effect of the differences in incubation time or incubation temperature on the results as seen in methods using resin, was not observed.
4) As the most linear and accurate range of the standard curve was between 2.5 and 10.0μg thyroxine iodine per 100 ml, it was necessary to determine the specimens by increasing the amount of serum with lower concentration of thyroxine iodine than 2.5μg per 100 ml and by decreasing the amount of serum with higher concentration of thyroxine iodine than 10.0μg per 100 ml.
5) A satisfactory reproducibility of the kit was obtained in measuring the serum from the subjects with various thyroid functions and from the pregnant women.
6) The values of the thyroxine iodine concentration (μg/100 ml) ranged 5.53±1.30 in normal subjects, 15.40±3.77 in patients with hyperthyroidism, 1.64±1.21 in patients with hypothyroidism, and 8.07±1.87 in pregnant women. A good correlation between the value of Tetrasorb and that of Tetralute was observed.
From these observations, it was concluded that the Tetralute test was useful screening technique for thyroid function with its simplicity and accuracy, although it was troublesome to change the volume of specimens with lower or higher concentration of thyroxine iodine than 2.5 or 10.0μg/100 ml.

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Purity Test of Iodinated (¹²⁵l) Insulin Solution, Iodinated (¹²⁵l) IgE Solution and Iodinated (¹³¹l) PVP Injection

Studies on the Quality and Test Method of Radiopharmaceuticals (4)

Goro URAKUBO, Kunisuke NAGAMATSU, Hideharu IKEBUCHI

1974Volume 23Issue 6 Pages 342-346
Published: June 15, 1974
Released on J-STAGE: September 07, 2010

DOIhttps://doi.org/10.3769/radioisotopes.23.6_342

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Gel filtration with thin layer made of Sephadex was applied to the purity test of 3 kinds of radiopharmaceuticals containing iodinated (¹²⁵Ior¹³¹I) high molecular substances.
Sephadex G-50, Superfine was used to make thin layer for iodinated (¹²⁵I) insulin solution and iodinated (¹³¹I) polyvinylpyrrolidone injection, and Sephadex G-200, Superfine was used for iodinated (¹²⁵I) IgE solution, according to the molecular weights of these radiopharmaceuticals.
Drops of these radiopharmaceuticals were spotted on wet thin layer plates and developed by declining technique with water for 1.5-5 hours. The distribution of radioactivities on these chromatograms was measured, and a sharp peak corresponding to the pure iodinated (¹²⁵Ior¹³¹I) substance was observed in each case.
So, this gel filtration procedure was regarded as a suitable method for purity test of these radiopharmaceuticals, detecting radioactivities due to possible degradation product and free iodine.

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A Mössbauer Study of Mixed Valence Iron Fluorides

Takuhiko SAKAI, Takeshi TOMINAGA

1974Volume 23Issue 6 Pages 347-348
Published: June 15, 1974
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.23.6_347

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The Simultaneous Measurement of Australia-Antigen and Antibody by Solid-Phase Radioimmunoassay Method

Tadamichi MASUOKA, Sigeharu MIMOTO

1974Volume 23Issue 6 Pages 349-352
Published: June 15, 1974
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.23.6_349

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Autoradiographic Study of the DNA Synthesis in Rat Liver after Severe Subtotal Hepatectomy; with Special Reference to the Lag Period in the Utilization of ³H-Thymidine and ³H-Deoxycytidine

Ayako UEKI, Hartmut RABES

1974Volume 23Issue 6 Pages 353-354
Published: June 15, 1974
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.23.6_353

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Activation Analysis of Environmental Pollutants

Tetsuo MAMURO

1974Volume 23Issue 6 Pages 355-363
Published: June 15, 1974
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.23.6_355

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Safe Handling of Unsealed Radioisotopes (VI)

Field Work (1) Factory Experiments

Kunio NISHIMURA

1974Volume 23Issue 6 Pages 364-366
Published: June 15, 1974
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.23.6_364

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Field Work (2) Agricultural Engineering

Toshiro OCHIAI

1974Volume 23Issue 6 Pages 367-369
Published: June 15, 1974
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.23.6_367

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Field Work (3) Field Experiment on Rice Plant Using ³²P

Masao SHIBUYA

1974Volume 23Issue 6 Pages 369-371
Published: June 15, 1974
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.23.6_369

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[title in Japanese]

1974Volume 23Issue 6 Pages A1443-A996
Published: June 15, 1974
Released on J-STAGE: July 21, 2010

DOIhttps://doi.org/10.3769/radioisotopes.23.6_A1443

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