1983 Volume 32 Issue 10 Pages 485-490
This study was undertaken to investigate the accumulation of 67Ga in an experimental abscess and to elucidate the mechanism of 67Ga uptake in the abscess.
Two, three, five, seven and ten days after subcutaneous injection of 0.2ml turpentine to the rats, 67Ga-citrate was injected to the rats. Twenty-four hours after injection of 67Ga, abscess and organs were excised and uptake rates of 67Ga were assayed. Furthermore, five days after subcutaneous injection of 0.2ml turpentine to the rats, 67Ga-citrate was injected to the rats, at various time intervals from 10 minutes to 6 days, abscess and organs were excised and uptake rates of 67Ga were assayed.
And subcellular distribution of 67Ga in abscess was determined at various time intervals after administration of 67Ga-citrate. On the other hand, to elucidate 67Ga binding substances in abscess, 67Ga-citrate and sodium sulfate-35S were injected to the above rats, respectively. Twenty-four hours after injection, abscess was excised and homogenized. The homogenate was digested with proteinase. After digestion, the reaction mixture was gelfiltered on Sephadex G-100. Eluate samples were assayed for radioactivity, uronic acid and protein.
Uptake rates of 67Ga in abscess increased with time after injection of turpentine and reached a plateau 5-7 days later. Ten minutes, 24 hours and 72 hours after injection of 67Ga, uptake rates of 67Ga in abscess were 0.92%/g, 3.3%/g and 5.6%/g, respectively. Uptake rates of 67Ga (24 hours after injection) in abscess was 2.0-3.4 time of tumor uptake rates (previously reported) . And, large amounts of 67Ga were in supernatant and decreased with time, but 67Ga in lysosome increased with time until 24 hours.
On the other hand, 67Ga was eluted with acid mucopolysaccharides with molecular weights of 9400-40000, and with molecular weight larger than 40000. And, it was concluded that 67Ga was bound to the acid mucopolysaccharides (heparan sulfate, etc) in abscess.
