植物体中微量代謝成分の¹³C濃度の赤外線¹³CO₂分析計による測定

抄録

The infrared ¹³CO² analyzer was improved to measure the ¹³C abundance in the trace amounts of plant metabolites separated by high performance liquid chromatography (HPLC) . To improve the sensitivity to ¹²C, the range of wavenumber of 2 360 ± 10 cm^-1 was used for ¹²C determination.
Free carbohydrates in corn leaves fed ¹³CO₂ were separated by HPLC and the ¹³C abundances in sucrose, glucose and fructose were determined by this improved method. The samples containing 10-40, μg of carbon could be successfully analysed.