Abstract
We have reported fundamental studies on the TSH immunoradiometric assay, using TSH RIABEAD II kit (Dainabot) . The sensitivity of the assay was 0.03μIU/ml and its C. V. was 27.2%. Intra-and inter-assay C. V. were less than 5%. Dilution test and recovery test were good. Serum TSH level was 0.3-4.0μIU/ml in normal subjects, less than 0.03μIU/ml in untreated Graves' disease and subacute thyroiditis. Therefore, it was found that the clear difference exist in serum TSH levels between normal subjects and patients with untreated Graves' disease. There was a well correlation on the serum TSH levels between this method and TSH radioimmunoassay kit (Amerlex TSH, r=0.983) . Especially, the measurement of serum TSH levels, using Immunoradiometric assay kit, was useful for the diagnosis of patients with Graves' disease.
