抄録
Repeated intramuscular injection of folic acid and ceruloplasmin results in appearance of erythropoietic activity in rabbit plasma as early as the fifth day after the injection. Although the combined use of folic acid and ceruloplasmin increased activity in blood, folic acid or ceruloplasmin alone; Vitamin B12 and ceruloplasmin; or Vitamin B12 alone failed to increase activity in blood. These value of Fe59 incorporation rate were two to three times that of the control. This activity can be shown at any time after one week following the intramuscular injection. This activity was not increased by substituting γ-globulin for ceruloplasmin which seems to suggest that ceruloplasmin has some role in the formation of erythropoietic activity. Erythropoietic activity described above was assayed by measuring the incorporation of Fe59 into peripheral red blood cells of pure strain Wistar rats.
Incorporation of Fe59 into protoporphyrin was markedly accelerated in the presence of N5-methyltetrahydrofolic acid and ceruloplasmin. Its incorporation rate increased in parallel with the amount of N5-methyltetrahydrofolic acid. Ceruloplasmin; N5-methyl tetrahydrofolic acid; tetrahydrofolic acid; or folic acid alone have only slight and somewhat variable activity in iron incorporation, while copper iron has no activity.
In hypoplastic anemia, serum ceruloplasmin levels were ranged from 10 mg./dl. to 60 mg./dl.. Gendel recommended a trial of folic acid using large doses over a long period of time. It is inferred that administration of folic acid using large doses is effective in the case of hypoplastic anemia with high serum ceruloplasmin level over 40 mg./dl..
