ガラスビーズへの血小板粘着について

—走査型電顕による検討—

抄録

Four ml of native whole blood and whole blood with anticoagulants, adenosine, AMP or aspirin were passed through the columns manually at a flow rate of one ml per 10 sec., and effluent blood was collected in four aliquots of 1 ml each.
Not only adhering but also aggregation of platelets were detected on the surface of glass beads even after first ml of native whole blood were passed through the columns. The more whole blood were passed through the column, the more platelets were retained by the column by means of their aggregation on glass surfaces. When glass heads were washed with 10 mM EDTA saline, aggregated platelets disappeared and only same adhered platelets remained on the surface.
Decalcification of whole blood, or adding of adenosine or AMP to the native whole blood, almost inhibited platelet retention by means of peventing platelet aggregation on glass surface.
Aspirin which was thought to inhibit secondary platelet aggregation due to ADP, epinephrine or collagen, in vivo or in vitro, had no effect on the retention rate of platelets.
Retention of platelets by glass bead columns appeared to be determined by platelet adhesion to glass surface and platelet aggregation due to exogenous ADP, which induced no secondary aggregation of platelets.