Largo法によるsoluble fibrin測定の検討およびStaphylococcal Clumping Testとの比較

抄録

A new method for detecting small amount of soluble fibrin was reported by Largo et al in 1976, which was visualized by an agglutination reaction of glutaraldehydetreated human erythrocytes coated with purified fibrin monomer. We examined the test conditions of Largo's method and compared it with staphylococcal clumping test (SCT) and hemagglutination inhibition test (HIT) in vivo as well as in vitro. An agglutination time was influenced by the degree of purification of fibrin monomer and by the amount of coated fibrin monomer, less influenced by fibrinogen and FDP. This method showed a sensitivity for fibrin monomer and soluble fibrin, whereas SCT was sensitive for fibrinogen, early FDP, fibrin monomer and soluble fibrin. HIT was not sensitive for detecting fibrin monomer and soluble fibrin. In various diseases such as nephritis, DIC, liver cirrhosis, hepatitis, coronary diseases, thrombosis and diabetes mellitus, soluble fibrin was detected in plasma by Largo's method. Especially nephritis was characterized by both high levels of SCT and large amount of soluble fibrin measured by Largo's method.