A 53 year-old female was suffered from acute hepatitis from the end of October to the begining of December, 1977. She noticed purpura on December 31, 1977 and was admitted to Nagoya 1st Red Cross Hospital on January 4, 1978. Severe aplastic anemia was diagnosed with hematological investigations as follows: granulocyte count 396/cmm, Reticulocyte count 15,600/cmm, platelet count 16,000/cmm in peripheral blood and 27.2% hemopoietic cells in bone marrow.
Prior to bone marrow transplantation, she was conditioned for grafting with cyclophosphamide 50mg/kg on each of four successive days. Bone marrow cells (1.13×1010) from her HLA-A, B matched and MLC negative sister (37 year-old) were infused intravenously 48 hours after the last dose of cyclophosphamide. She received intermittent methotrexate therapy after grafting to modify graft-versus-host disease (GVHD). On 12th day after bone marrow transplantation, hemopoietic cells and CFU-c in bone marrow increased in number, 80.8% and 54/2×105 cells respectively. Peripheral blood cell elements gradually increased too. Her red blood cell type (CEcS) changed to the donor type on day 68. On 85th day after marrow grafting, skin eruption (GVHD) appeard over whole body and lasted for about one and a half month.
She is alive in good hematological condition over one year after bone marrow transplantation.
