臨床研究
ヒト多核白血球のchemiluminescence測定の基礎的検討
1979 年 20 巻 4 号 p. 372-380
詳細
1979 年 20 巻 4 号 p. 372-380
In 1972 Allen et al. reported that human polymorphonuclear leukocytes (PMNs) emitted chemiluminescence (CL) during the process of phagocytosis. After that CL response has been used as one of the examination techniques of PMNs function. But this technique is not yet widely used in Japan.
The author examined the methodological studies of CL response of human PMNs with a liquid scintillation spectrometor (Aloka-LSC 602) and has found that this method is simple, objective, reproducible and economical. Furthermore, this method is useful for screening PMNs abnormalities because the transitional changes of the CL response can be observed.
By using patients' serum instead of the pooled one, it was possible to know the patients' serum opsonic activity.
CL response of PMNs of chronic granulomatous disease (CGD) showed a characteristic pattern in which cpm did not elevate, and the CL response of its carrier was intermediate. So this method is valuable for the diagnosis of CGD and its carrier.
Also CL response is useful for the study of bactericidal mechanism of PMNs.
