Abstract
Euglobulin clot lysis time (ELT) has been said to be one of the most reliable methods for measuring fibrinolytic activity. Recently, Cl-Inactivator (Cl-INH) was identified as the only potent plasmin inhibitor in the euglobulin fraction, the presence of which had been neglected before. It is known that Cl-INH inhibits enzymes of the complement, kinin forming, coagulation and fibrinolytic systems.
The present studies were undertaken to evaluate the inhibitory activity of Cl-INH on the ELT. Assay of 201 clinical cases revealed significant positive correlation between the concentration of Cl-INH in the euglobulin fraction and ELT. Furthermore, the concentration of this inhibitor increased in various diseases.
In experimental studies, the effects of Cl-INH on ELT and kaolin activated ELT were investigated using the Cl-INH deficient plasma prepared by the method of antibody neutralization from the normal pooled plasma and factor XII deficient plasma. Lineality of dose-response curve was obtained between the Cl-INH and ELT as well as kaolin activated ELT. It was suggested that the Cl-INH influences ELT as an inhibitor of both plasmin and factor XIIa.
