再生不良性貧血の骨髄CFU-Eコロニー形成に対するFluoxymesteroneのIn vitro添加効果

抄録

We have investigated the in vitro effects of fluoxymesterone on erythroid colony growth from bone marrow of eight normal subjects and sixteen patients with aplastic anemia. Seven patients were androgen dependent, three were androgen effective, and six were androgen ineffective by previous treatment. CFU-E assay was carried out using the plasma clot method with 2×10⁵ marrow cells/dish, 1.0 U/ml of erythropoietin, and various concentrations of fluoxymesterone.
It was demonstrated that the number of erythroid colonies from marrow of androgen dependent patients was significantly increased with the addition of fluoxymesterone in comparison with that of normal subjects. On the other hand, the number of erythroid colonies from marrow of androgen ineffective patients was not significantly increased compared to that of normal subjects.
The results may suggest that there exist unique CFU-E clones which are very sensitive to androgens in vitro as well as in vivo in the patients with androgen dependent aplastic anemia. In addition, it has been revealed that in vitro fluoxymesterone sensitivity test may be a valuable indicator to predict the response to androgen therapy in the patients with aplastic anemia.