小児急性リンパ性白血病におけるDNA aneuploidyの臨床的意義の検討

抄録

Using flow cytometric techniques, we determined the pretreatment distribution of DNA content in leukemic blasts from 72 children with acute lymphoblastic leukemia (ALL). Abnormal DNA stem lines (DNA aneuploidies) were detected in 23 cases (31.9%) and the DNA index (DI) ranged from 1.04 to 1.41. Surface phenotype defined by J-5 monoclonal antibody and age were significantly related to DNA aneuploidy. Treatment response, measured as the continuous time of first remission, was assessed for 36 cases: DI>1 (n=11) and DI=1 (n=25). When the continuous remission curves were compared, the aneuploid cases with DI>1 showed better response than the diploid cases with DI=1. Results of a multivariate analysis indicated that DNA content, as well as other clinical and biological features (WBC count, age and surface phenotype), was a strong predictor of patients' outcome.