シンポジウム2．
脾臓と赤血球
脾臓と赤血球
脾と造血
1989 年 30 巻 8 号 p. 1239-1243
詳細
1989 年 30 巻 8 号 p. 1239-1243
In order to investigate the role of the human spleen on hematopoiesis, hematopoietic stem cells and stimulaters were evaluated in fetal and adult spleens. BFU-E and CFU-C were existed in 20 weeks and 23 weeks fetal spleens (BFU-E 145±45/105 mononuclear cells, CFU-C 55±6/105 mononuclear cells). In adult spleen, a few stem cells were recognized, which may be contaminated from peripheral blood in sinus of the spleen. We tested conditioned media from adult spleen cells for the stimulative activity on the in vitro growth of BFU-E and CFU-C from bone marrow mononuclear cells. Spleen conditioned medium stimulated proliferation of these precursor cells. It seemed that PHA-stimulated spleen conditioned medium augmented BFU-E, whereas CFU-C growth was suppressed. Adult and fetal spleens were studied immunohistochemically using anti-G-CSF, GM-CSF and erythropoietin anti-bodies. The cells with G-CSF and GM-CSF were shown in fetal spleens. In adult spleens, however, only GM-CSF was detected.
