抄録
It has been previously reported that some fragile cells were found in reticulocytes which appeared after massive hemorrhage.
The present studies were made on the phenomenon and mechanism of the early disappearance of abnormal reticulocytes.
From the change in the peripheral erythrocyte indicies after massive hemorrhage, the abnormal reticulocytes or “stress reticulocytes”, which were suggested by us to be formed by the early denucleation of intermediate erythroblasts, were thought to belong to large size cells with a low hemoglobin concentration.
As these macroerythrocytes were confirmed to be earsily sequestered in sinuses of the RES organs, the influences on the cells and the changes in media were studied under sequestration.
During the in vitro 37°C incubation of reticulocyte-rich blood, decreases were observed both in pH of media and in reticulocyte count.
Under similar in vitro stasis, increases of osmotic fragility and autohemolysis were found in the newly formed erythrocytes due to bleeding.
These immature cells seem to be much more influenced by some metabolic inhibitor, but they are resistant to mechanical fragility tests.
The above data, as summarized, suggest that stress reticulocytes are easily sequestered in sinuses and are more selectively conditioned by factors during sequestration resulting in their early destruction.
