アミノ酸（ピバロイル／イソブチルエステル誘導体）の GC/MS による解析

抄録

We report an analytical procedure of gas chromatography / mass spectrometry (GC / MS) focusing on chromatographic enantiomer separations and the corresponding mass spectra. We used N-pivaloyl isobutyl esters of amino acids to show the summary of retention time and those of mass fragmentation patters. To avoid matrix effect from biogeochemical samples, we optimized the entire experimental flow showing an optional wet chemical treatment for the purification of amino acids. We separated both of protein and non-protein type amino acids derived from the samples, including prokaryotic soft-tissues, freshwater / marine biological hard-tissues, and a deep-sea serpentine sample. Present derivatization procedures of amino acids as pivaloyl / isobutyl esters can be switchable on the diastereomers with the use of S-(+)-2-butyl esters or R-(-)-2-butyl esters. The present method will be applied into a further description of enantiomer-specific isotopic composition of individual amino acid.