イオンペアクロマトグラフィー／電子スプレーイオン化質量分析法 （LC/ESI-MS）による短鎖ペプチド類のマススペクトル解析
2021 年 37 巻 1 号 p. 15-43
詳細
2021 年 37 巻 1 号 p. 15-43
We report a mass spectra database of underivatized short-chain peptides by using ion-pair liquid chromatography with electrospray ionization-mass spectrometry (LC/ESI-MS). The ion-pairing chromatographic separation by a porous graphitic carbon stationary column with nonafluoropentanoic acid solution and acetonitrile solvent gradient revealed a positive correlation between the retention time and the degree of molecular hydrophobicity. The addition of a proton donor of 0.1% formic acid for the inverse gradient program induces an efficient stabilized ionization on the ESI positive mode. We compiled the summary of retention time and those of some typical mass fragmentation patterns on underivatized short-chain peptides and related-molecule groups. The present method will be applied to other multi-dimensional chromatographies for further detailed description of short-chain peptide chemistry.
