ヒト卵管上皮細胞の無血清培養法の確立とその免疫組織学的および超微形態学的研究

抄録

With the widespread use of in vitro fertilization-embryo transfer, the oviduct continues to play an important role in successful pregnancy. While the exact effects of the oviduct on the reproductive process remain unclear, steroid hormones, especially estrogens, have been demonstrated in vivo to induce and maintain the maturation and secretory activities of oviduct epithelial cells. However, it has usually been difficult to observe the effects of estrogens on the oviduct epithelial cells in experimental systems, because the addition of estrogens to the culture medium containing serum usually induced the proliferation only of stromal cells but not of epithelial cells. In this study a part of human oviduct new culture model has been applied to epithelial cells obtaind from ampulla and the established cells cultured have been studied immunohistochemically and ultrastructurally as well.
After 3 weeks of culture in serum-free medium, most of the cells revealed positive reaction in keratine and epithelial membrane antigen (EMA) stainings, and negative in vimentin. However, the cells cultured in the medium containing serum or calcium were exclusively positive in vimentin reaction.
Oviduct epithelial cells are composed mainly of secretory and ciliated cells. The epithelial cells cultured on a collagen gel base with serum-free medium showed ultrastructural characteristics similar to those of secretory cells of the oviduct epithelium, and the cells in the over layered medium contained ciliated cells.
Both of ciliated and secretory cells cultured in the floating collagen with serum-free medium proliferated in the similar way of cellular arrangement as in oviducts epithelium on the floating collagen gel.
Estrogen and progesterone receptors were immunohistochemically observed in the epithelium of oviduct as well as in cultured epithelial cells were also.
With the use of estrogen in the serum-free medium, the cultured epithelial cells showed a marked increase of cytoplasmic organella and the surface protrusions suggesting apocrine secretion were frequently obserbed.
However, proliferation of cultured cells was not accelerated by the addition of estrogen (E₁, E₂ or E₃) or progesterone to the medium. [Adv Obster Gynecol 47(2) : 261-276, 1995 (H7.3)]