抄録
Phos-tag serves as an analytical tool for selective enrichment of phosphorylated molecules. Recent technological advances regarding mass spectrometry-based quantitative proteomics, in combination with Phos-tag technology, have enabled us to describe the comprehensive status of phosphorylated signaling molecules in a time-resolved manner. Integrative analysis of phosphoproteome dynamics with transcriptome regulation in breast cancer cells uncovered core network hubs related to drug resistance at the system level.
