6.マイクロ電気泳動装置

抄録

Micro-disc electrophoresis is a powerful tool for high resolution micro-analysis of brain secreted proteins and peptides of land snail, Euhadra peliomphala.
Micro-disc electrophoresis apparatus consists of two parts, that is, cylindrical gel electrophoresis chamber with gel tubes (50mm long, 0.8mm inside diameter) and regulated power supply with maximum voltage and current of 500V and 500μA, respectively.
High resolution gel and buffer system for micro-disc electrophoresis is also developed.
High resolution electrophoretic pattern of brain secreted proteins was obtained only by electrophoresis under the constant current of 40μA/tube.
Brain specific peptide (5, 800 dalton) of Euhadra appeared only in adult brain.