無担体電気泳動装置における等電点電気泳動の基礎条件の検討
1983 年 27 巻 3 号 p. 131-138
詳細
1983 年 27 巻 3 号 p. 131-138
A new type apparatus was designed for carrier free isoelectric focusing which could be connected to high-performance liquid chromatography. The merits of this apparatus are as follows:
1) The electroosmosis was low due to the plastic quality of electrophoresis tubing instead of the glass tubing.
2) An amount of sample could be increased by increasing the number of tubings for electrophoresis.
3) For fractionation, this apparatus was connected by pumping-out device with peristaltic pump to a UV detector and a fraction collector.
For simplification and quick operation of this apparatus, we studied on basic conditions and on the separation of proteins by isoelectric focusing using carrier free electrophoresis. With incorporation of prefocusing process at high voltage, whole operation time could be shortened to one hour or less.
