PAG electrophoretic patterns of human sweats

Abstract

Sweats were obtained from 54 healthy adult men in a sauna. Supernatants of the sweats after centrifugation were dialyzed with deionized water and lyopholized. The sweat samples dissolved in normal saline were tested by PAG-slab electrophoresis. The sweat samples obtained from the 52 men were stained with Coomassie brilliant blue R250 and those from the 54 men were with PAS. The results were as follows: All the sweat samples had one band corresponding with the electrophoretic mobility of serum albumin. Two bands seemed to be important components of sweats were in the α-globulin region of serum. Individual differences of electrophoretic patterns of the sweat samples were found in the regions of α-, β- and γ-globulins. Three glycoprotein components having a individually different mobility were observed in the pre-albumin region.