PAGIEFゲルの両面からニトロセルロース膜への蛋白の転写 (両面転写)

抄録

A new method has been devised for the electrophoretic transfer of proteins from the PAGIEF gel to a pair of nitrocellulose membranes which placed on the both sides of the gel. With repeatedly changing over the polarities of electrodes of the apparatus in accordance with the timetable, this method enabled to equally transfer the proteins to a pair of nitrocellulose membranes simultaneously. Furthermore, it was possible to equally quantitatively transfer the proteins of molecular weight about 50, 000∼120, 000 to two pairs of nitrocellulose membranes.
The results indicate that the both-sides blotting is applicable in the studies of genetic polymorphism of plasma proteins and identification of protein antigens following two-dimensional gel electrophoresis.