免疫化学的手法を用いて分画されるU1-およびU2-snRNP
1988 年 32 巻 1 号 p. 23-26
詳細
1988 年 32 巻 1 号 p. 23-26
We obtained a new SLE-autoantibody affinity column which can fractionate U1- and U2-snRNPs simultaneously. The purified human U1- and U2-snRNPs were recovered from the column by elution with 2.5M MgCl2 at pH7.2 after the column had been washed with Tris-buffer containing 1.0M NaCl. Highly purified U1- and U2-snRNP particles were also obtained from the mouse liver (129/sv) and feline placenta, by the same procedure as used for the purification of human KB cell U1- and U2-snRNPs. By UV spectrum analysis, the materials purified from the column was suggested to form the ribonucleoprotein particles.
