抄録
The use of automated devices in the last ten years have made biomedical applications of cell electrophoresis more attractive. We are using routinely the automated single cell electrophoresis microscope PARMOQUANT. Using the PARMOQUANT we applied this method to discriminate lymphocytes and study the interaction of substances with cells and synthetic particles. Electrophoretic histograms allowed the determination of changes in the proportion of lymphocyte populations after kidney transplantation, during dialysis treatment, open heart surgery and during pregnancy. Discrimination of leukemic cells on the basis of electrophoresis was used as an additional parameter in diagnosis. In a mouse tumor model histograms determination enabled evaluation of the in vivo effect of tumor necrosis factor on immune cells. Cell electrophoresis was shown to be suitable to detect the influence of antibodies, lectins and bacteria on the cell surface. Protein adsorption was studied on synthetic particle using cell electrophoresis. This method was applied in investigating the phenomena of blood interaction with biomaterials for use in artificial organs and to determine differences in the protein composition in serum or other body fluids connected with diseases. On the basis of this principle a test to detect hetrozygotes in cystic fibrosis is now in progress.
