1977 年 11 巻 2 号 p. 12-16
Extract conditions of urocanic acid, a metabolic substance of histidine in the human epidermis, from the stratum corneum with water, and transepidermal of urocanic acid free stratum corneum were studied.
A new quantitative analytical method of urocanic acid by High Speed Liquid Chromatography was developed. Toyosoda LS-140 was employed as column packing. This method is more acurate and more rapid than conventional Thin Layer Chromatography. Extraction of urocanic acid from stratum corneum was perfomed by the cup method.
Urocanic acid was shawn to be rapidly eluted into water from stratum corneum and to be reproduced at a slow pace.
Consequently, stratum corneum once extracted with water was retained longer under urocanic acid deficient state than the control stratum corneum.