抄録
Alkaline phosphatase was microcapsulated to be immobilized with stylene polymer by employing a drying in liquid method. The size distribution of the microcapsules was a bimodal form. The larger microcapsules had the stronger activities and were the more dense than the one with the smaller size. The optimum temperature and pH for the reaction of the microencapsulated enzyme shifted to the higher ranges than the native enzyme. The reaction rate of the microcapsules because delayed significantly. The reaction process was analyzed by Lineweaver-Burk plots of data to test the Michael-Menten equation. It was found that the reaction rate was controlled by not only the diffusion of donor in the microcapsule but also a cappillary flow of the enzyme solution through the pin holes on the surface of the microcapsule, which were observed by a scanning electron microscope. The latter was proved by the fact that the reaction rate was affected by agitation speed and the reaction proceeded still more even if the microcasules were removed from the system.
