プラセンターリキッド中のシアル酸の検出

抄録

We tried to detect Sialic acid in Placentaliquid (PL) obtained from bovine-placenta and presence of N-acetylneuramic acid (NANA) as one form of Sialic acid has been predicted.
After separation with paper-chromatography, ketoamino sugar acids were identified as NANA by absorption spectrum of colored substance with periodate-thiobarbituric acid and resorcinol-Cu²⁺-HCl reagents.
We substituted the determined value of ketoamino sugar acids from the color reaction for Sialic acid content in PL. This method will be useful both as a new method of evaluation of natural extracts predicted to contain Sialic acid and as study of bioactivity of theextracts.