抄録
Chromosomal behaviour following mutagenic treatments was studied in M1 generation of chickpea, var. Pusa-212 and Pusa-372. A wide spectrum of meiotic aberrations was induced in the treated populations, the most frequent being stickiness of chromosomes, univalents, multivalents, laggards, bridges, cytomixis and disturbed polarity. Other anomalies such as non-orientation of bivalents, precocious separation, non-disjunction and unequal segregation of chromosomes were also recorded but comparatively in less frequency. The frequency of meiotic aberrations and pollen sterility increased with the increase in dose/conc. of the mutagen. Combination treatments were found to be most effective than separate treatments of gamma rays and ethyl methanesulfonate (EMS) in inducing maximum frequency of meiotic aberrations. Among various stages of meiosis, frequency of metaphase anomalies was more followed by anaphase and telophase. The coefficient of interaction for various combination treatments was less than additive with respect to the overall frequency of meiotic aberrations and pollen sterility. In general, var. Pusa-372 was more sensitive to mutagenic treatments than var. Pusa-212. The present findings reveal the potential of gamma rays, EMS and their combinations in inducing mutations and enhancing genetic variability in chickpea for isolation of economically important mutations in the segregating generations.
