抄録
Four cestode samples collected from four patients were identified by homology searching of the partial mitochondrial cytochrome oxidase subunit 1 gene (COX1) sequences. The COX1 region was amplified by PCR using a pair of JB3 and JB4.5 or DBCOI-1 and DBCOI-2 primers for the Pseudophyllidea cestode and by multiplex PCR for the Cyclophyllidea cestode. The COX1 sequences of two samples speculated to be Diphyllobothrium nihonkaiense and D. latum were morphologically identical and homology searching showed that the sequences of the two samples had 99.6% to 100% identity with those of D. nihonkaiense. The two samples were therefore identified as D. nihonkaiense. Similarly, a sequence from a larva isolated from the great omentum of one patient showed 98% to 100% identity with that of Spirometra erinaceieuropaei. This sample was therefore identified as larval S. erinaceieuropaei. The remaining sample tested positive for Taenia saginata in multiplex PCR and the sequence from the diagnostic fragment had 99.7% to 100% identity with that of T. saginata. This sample was therefore identified as T. saginata. The present findings highlight the usefulness of genetic examination for the diagnosis of cestodiasis.
