2017 Volume 69 Issue 3 Pages 123-126
Three-dimensional （3D） in vitro microvasculature in a microdevice was developed as a physiologically relevant model of angiogenesis. The angiogenic process was monitored using stage-top optical coherence tomography （OCT）. The OCT allows non-invasive monitoring of the 3D structures of the prepared host microvasculature and sprouted neovasculature without fluorescence staining. The obtained OCT cross-sectional images capture 3D features of the angiogenic sprouting process and provide information on the dynamics of luminal formation. The stage-top OCT system is a simple and convenient monitoring method that enables to visualize the in vitro dynamics and morphological changes of 3D cultured cells in live.
