Natural abundance of 15N (δ15N) in nitrogen compounds in forest ecosystems is a useful parameter for analyzing the nitrogen cycle in forests. The δ15N value for nitrogen compounds is determined mainly by input of N (N-fixation and precipitation) originating from atmospheric N2 less enrichment with 15N and output of N through denitrification and nitrate leaching, which leads to preferential 15N enrichment in ecosystems. Temporal and spatial variation in δ15N can provide useful information on changes in nitrogen cycling in terrestrial ecosystems. Some case studies using this technique are reviewed.