Immobilization of hydrogenase using polymer containing cationic side chains

Abstract

Enzymes, which catalyze a variety of reaction in living systems, have many specific features, such as high reactivity and safety for the environmental. However, enzymes are relatively expensive, and therefore discarding them after a single use is not economical. A variety of methods for the immobilization of enzymes have been eveloped, but strong interaction between enzyme and carrier causes denaturalization and deactivation of enzymes. In this work, we report a method of hydrogenase immobilization using polymer containing cationic side chains. Hydrogenases catalyze evolution hydrogen by proton reduction. This method prevented denaturalization of enzymes by using electrostatic interaction.