大阪歯科学会例会抄録
2 ヒト口腔扁平上皮癌に対するモノクローナル抗体の作製とその診断学的応用 (第407回 大阪歯科学会例会)
1991 年 54 巻 6 号 p. 532-533
詳細
1991 年 54 巻 6 号 p. 532-533
Three monoclonal antibodies (MoAbs) NG3, NZ9, and Z17 were produced from electric cell fusion of mouse myeloma cell line NS-1 with spleen cells from BALB/c mice that had been immunized with transplanted squamous cell carcinoma (SCC) from nude mice (TSG1, TSZ2) or immunized with a control tongue tumor. By immunohistochemistry, NG3 (IgM, ^K-chain) and NZ9 (IgG1, ^K-chain) showed strong immunoreaction with the cellular cytoplasm and membrane of moderately and well differentiated SCC, respectively. Z17 (IgM, ^K-chain) strongly immunostained the cellular cytoplasms of poorly differentiated SCC. However, none of the three MoAbs stained the stroma. NG3, NZ9 and Z17 were useful in diagnosing SCC, and in classifying its differentiation.
