3 パラフィン切片におけるヒト口腔癌のHPV DNA高感度検出(第497回 大阪歯科学会例会)

抄録

Although human papillomavirus (HPV) infection has been shown to be a significant carcinogen in cervical squamous cell carcinoma (SCC), its significance in oral SCC remains unclear. We developed highly sensitive detection methods for HPV to elucidate the prevalence and localization of HPV in paraffin sections from human oral SCC using modified in situ PCR and in situ hybridization AT tailing (ISH-AT). Analyses revealed a high prevalence of several HPV types (HPV-16, 18, 22, 38 and 70) under optimal conditions. The ISH-AT method can be used as an alternative to in situ PCR. Various staining patterns were observed in the 20 cases and HPV-positive cells were localized within the surface epithelium as well as in neoplastic cells. We demonstrated that HPV DNA could be detected in paraffin sections using either the method of in situ PCR or ISH, providing an appropriate primer and probe are used.